Multicolor spectral karyotyping allows simultaneous visualization of all hu
man chromosomes and screening for chromosomal rearrangements without a prio
ri knowledge of any abnormalities involved. Based on this potentially incre
ased sensitivity, we investigated, in a preliminary manner, whether spectra
l karyotyping could detect cytogenetic aberrations in karyotypically normal
leukemia. The test population was comprised of 28 cryopreserved, cytogenet
ically normal acute myeloid leukemia (AML) samples from patients registered
to a randomized trial for previously untreated AML (SWOC 9031). Two normal
and 12 samples with known cytogenetic aberrations were used to validate an
d establish the diagnostic accuracy of the spectral karyotyping assay and i
nstrumentation in a clinical setting. Enumeration and region-specific DNA f
luorescence in situ hybridization (FISH) probes verified discrepant results
. In the validation data set, spectral karyotyping refined complex karyotyp
ic rearrangements in six cases and defined the chromosomal origin of a "jum
ping" homogeneously staining region; however, the technology was less sensi
tive in the detection of subtelomeric rearrangements and double minute chro
mosomes. In the test population, spectral karyotyping identified previously
undetected cytogenetic aberrations In two cases (7%) of karyotypically nor
mal AML: a cryptic 11q23 translocation in 20/20 cells and a minor monosomy
7 clone in 3/21 cells (FISH, 10.5%). Both of these abnormalities are consid
ered to confer a poor prognosis when based on classical cytogenetic prognos
tic criteria. As an adjunct to classical cytogenetics and standard FISH ana
lyses, the additive resolution of spectral karyotyping, in particular, with
chromosome paints spiked with subtelomeric and/or locus-specific probes, m
ay allow significant gains to be made in diagnostic accuracy and recognitio
n of genotype/phenotype prognostic relationships, and in defining underlyin
g biologic mechanisms in cancer. (C) 2000 Wiley-Liss, Inc.