A glucose transporter chimera confers a dominant negative glucose starvation phenotype in Saccharomyces cerevisiae

Authors
Sherwood, PW Katic, I Sanz, P Carlson, M
Citation
Pw. Sherwood et al., A glucose transporter chimera confers a dominant negative glucose starvation phenotype in Saccharomyces cerevisiae, GENETICS, 155(2), 2000, pp. 989-992
Citations number
24
Categorie Soggetti
Biology,"Molecular Biology & Genetics
Journal title
GENETICS
ISSN journal
00166731 → ACNP
Volume
155
Issue
2
Year of publication
2000
Pages
989 - 992
Database
ISI
SICI code
0016-6731(200006)155:2<989:AGTCCA>2.0.ZU;2-5
Abstract
A family of glucose transporters mediates glucose uptake in Saccharomyces c erevisiae. We show that the dominant mutation GSF4-1, which impairs glucose repression of SUC2, results in a nonfunctional chimera of the transporters Hxt1p and Hxt4p. Hxt1/4p inhibits the function of wild-type glucose transp orters. Similar mutations may facilitate analysis of the major facilitator superfamily.