P. Labonte et al., Inhibition of tumor growth with doxorubicin in a new orthotopically implanted human hepatocellular carcinoma model, HEPATOL RES, 18(1), 2000, pp. 72-85
A number of human xenograft orthotopic models of hepatocellular carcinoma (
HCC) have been previously established by growing histologically-intact pati
ent specimens in nude mice. However, the availability of HBV and HCV negati
ve human hepatocellular carcinoma specimens is scarce and the pattern of tu
mor growth in nude mice varies depending on the tumor type. In the present
study, we have established a reproducible xenograft orthotopic model using
a human HCC cell line designated HuT7-3 that was derived from two rounds of
subcloning of the parental Huh-7 cell line. The tumor growth rate of the H
uT7-3 cell line, grown at a primary subcutaneous site, was markedly higher
than that of the Huh-7 parental cell line or the human hepatoblastoma Hep-G
? cell line. Furthermore, we have shown that doxorubicin, when administered
intravenously, is efficient in inhibiting the development of subcutaneous
tumor but leads to the regression of the orthotopic human HCC. Consequently
, this novel HCC xenograft orthotopic model can be used for the evaluation
of antitumor drugs. (C) 2000 Elsevier Science Ireland Ltd. All rights reser
ved.