H-2/H-1 ratio analysis of flavor compounds by on-line gas chromatography pyrolysis isotope ratio mass spectrometry (HRGC-P-IRMS): Benzaldehyde

Isotope ratio monitoring gas chromatography-mass spectrometry of the H-2/H- 1 ratio by pyrolysis isotope ratio mass spectrometry (PIRMS) was used to an alyze benzaldehyde originating from various sources, Based on the delta(2)H (SMOW) value of an authentic reference sample determined with an elemental analyzer (EA), the range of reproducibility and linearity was checked. Corr ect (EA related) and reproducible data were obtained for sample amounts >0. 6 mu g benzaldehyde (on column). In another series of experiments, the infl uence of sample preparation, i.e. simultaneous distillation-extraction (SDE ) was found to be negligible, The following ranges of delta(2)H(SMOW) value s were determined for benzaldehyde using five types of samples, i.e. (i) sy nthetic (delta(2)H(SMOW) -78 to -85 parts per thousand, ex benzal chloride; +420 to +668 parts per thousand, ex toluene) and 'natural' (including 'ex- cassia') references (delta(2)H(SMOW) -83 to -1444 parts per thousand); (ii) bitter almond oils (delta(2)H(SMOW) -113 to -148 parts per thousand); (iii ) fruits (delta(2)H(SMOW) -111 to -146 parts per thousand); (iv) kernels (d elta(2)H(SMOW) -115 to 188 parts per thousand); and (v) leaves (delta(2)H(S MOW) - 165 to -189 parts per thousand).