Y-chromosomal STR haplotypes in a population sample from Bavaria

The seven Y-chromosomal STRs DYS19, DYS385 I/II, DYS389 I/II, DYS390, DYS39 1, DYS392 and DYS393 were amplified using two multiplex PCRs. The optimizat ion of the PCR conditions led to reliable and sensitive systems, Go-amplifi cation of the amelogenin locus was possible in both multiplex systems. In a population sample of 151 Bavarian males, a gene diversity of 0.99 was obse rved. Sensitivity studies revealed a detection limit of 50 pg DNA per 25 mu l reaction volume. PCR experiments with combinations of male/male and male /female DNA showed that in male/male mixtures, the minor component could be detected up to a ratio of 1:15, whereas in male/female mixtures the male c omponent could be found in a higher ratio up to 1:60.