A plant plasma membrane H+-ATPase expressed in yeast is activated by phosphorylation at its penultimate residue and binding of 14-3-3 regulatory proteins in the absence of fusicoccin

The Nicotiana plumbaginifolia plasma membrane H+ ATPase isoform PMA2, equip ped with a His, tag, was expressed in Saccharomyces cerevisiae and purified , Unexpectedly, a fraction of the purified tagged PMA2 associated with the two yeast 14-3-3 regulatory proteins, BMH1 and BMH2. This complex was forme d in vivo without treatment with fusicoccin, a fungal toxin known to stabil ize the equivalent complex in plants. When gel filtration chromatography wa s used to separate the free ATPase from the 14-3-3.H+-ATPase complex, the c omplexed ATPase was twice as active as the free form. Trypsin treatment of the complex released a smaller complex, composed of a 14-3-3 dimer and a fr agment from the PMA2 C-terminal region. The latter was identified by Edman degradation and mass spectrometry as the PMA2 C-terminal 57 residues, whose penultimate residue (Thr-955) was phosphorylated, In vitro dephosphorylati on of this C-terminal fragment prevented binding of 14-3-3 proteins, even i n the presence of fusicoccin, Mutation of Thr-955 to alanine, aspartate, or a stop codon prevented PMA2 from complementing the yeast H+-ATPase, These mutations were also introduced in an activated PMA2 mutant (Gln-14 --> Asp) characterized by a higher H+ pumping activity. Each mutation directly modi fying Thr-955 prevented 14-3-3 binding, decreased ATPase specific activity, and reduced yeast growth. We conclude that the phosphorylation of Thr-955 is required for 14-3-3 binding and that formation of the complex activates the enzyme.