Characterization of Cdk2-cyclin E complexes in plasma membrane and endosomes of liver parenchyma - Insulin-dependent regulation

Rat liver parenchyma Golgi/endosomes fractions harbor a tyrosine-phosphoryl ated 34-kDa protein. Screening of Golgi, endosomes (ENs), plasmalemma (PM), and cytosolic (Cyt) fractions revealed the presence of the mitotic kinase Cdk2 in ENs, PM and Cyt. The fluid phase endocytic marker horseradish perox idase gained access to the endosomal Cdk2, confirming its localization, Cdk 2 was shown to be associated to cyclin E and was active in ENs and PM fract ions. The administration of a single dose of insulin (1.5 mu g/100 g, body weight) induced a time-dependent activation of the insulin receptor kinase in these structures. Insulin receptor-kinase activation was followed by the inhibition of immunoprecipitated Cdk2-cyclin E kinase activity in PM and t he progressive disappearance of cyclin E. In marked contrast, no such effec t was observed in ENs. The injection of a phosphotyrosyl phosphatase inhibi tor (bpV-(phen)) increased the levels of cyclin E in ENs and PM. A massive recruitment of p27(hip1) was observed in the Cdk2-cyclin E complexes isolat ed from PM and Cyt but not from ENs. In vitro, Cdk2-cyclin E complexes have the capacity to inhibit the formation of hybrid structures containing hors eradish peroxidase and radioiodinated epidermal growth factor. Therefore, i n the PM and ENs of adult rat liver, an active and regulated pool of the mi totic kinase Cdk2-cyclin E and some yet to be defined effecters are present . Cdk2 may contribute to the modulation of transport events and/or maintena nce of the topology of endocytic elements.