Sensitive method for the determination of ambroxol in body fluids by capillary electrophoresis and fluorescence detection

A sensitive and rapid capillary electrophoretic method combined with laser- induced fluorescence detection has been developed for the determination of ambroxol. Samples were derivatized with 5.10(-4) M fluorescein isothiocyana te. A linear relationship between concentration and peak area was obtained in the concentration range 0.008-42 mu g ml(-1) with a correlation coeffici ent of 0.9999. The applicability of the method to serum and urine samples w as demonstrated. The method is also useful for the determination of ambroxo l in pharmaceutical preparations. (C) 2000 Elsevier Science B.V. All rights reserved.