Isolation of cDNA clones corresponding to genes expressed during fruit ripening in Japanese pear (Pyrus pyrifolia Nakai): involvement of the ethylenesignal transduction pathway in their expression

Thirty cDNA clones of genes corresponding to mRNAs up-regulated during frui t ripening of Japanese pear (Pyrus pyrifolia Nakai cv, Kikusui) were obtain ed by differential screening of a ripe fruit cDNA library. All of these cDN As were sequenced and gathered into 11 non-redundant groups after database searches. These clones represented genes associated with stress responses, protein catabolism or pathogenesis. The accumulation of transcripts of 3 ou t of 11 genes was inhibited by 1-methylcyclopropene (MCP), an inhibitor of ethylene action.