Characterization of Moroccan isolates of Verticillium dahliae Kleb using RAPD markers

Isolates of Verticillium dahliae were sampled from different olive tree orc hards in Morocco. These olive trees were located in different commercial cu lture locations in southern, central and northern Morocco. The isolates wer e characterized using genetic markers obtained after their DNA PCR amplific ation with random amplified polymorphic DNA (RAPD) primers. Among the 40 pr imers tested, 10 generated a total of 66 polymorphic fragments. Among the 3 8 isolates of V. dahliae tested, RAPD markers were successful in the charac terization of groups based on their geographic origin. With the exception o f one specific isolate, no correlation could be established among the isola tes, based on the morphological appearance of the colony in culture.