Identification of genes expressed in the epithelium of porcine oviduct containing early embryos at various stages of development

Citation
Hs. Chang et al., Identification of genes expressed in the epithelium of porcine oviduct containing early embryos at various stages of development, MOL REPROD, 56(3), 2000, pp. 331-335
Citations number
21
Categorie Soggetti
Cell & Developmental Biology
Journal title
MOLECULAR REPRODUCTION AND DEVELOPMENT
ISSN journal
1040452X → ACNP
Volume
56
Issue
3
Year of publication
2000
Pages
331 - 335
Database
ISI
SICI code
1040-452X(200007)56:3<331:IOGEIT>2.0.ZU;2-O
Abstract
As a first step toward elucidation of the action of factors secreted by the epithelium of oviduct, differential display reverse transcription-polymera se chain reaction (DDRT-PCR) was used in this study to identify transcripts of such oviductal factors in gifts carrying various stages of early embryo development post hormone-induced ovulation. A total of 13 differentially e xpressed transcripts were identified between 50 and 120 hr post-hCG injecti on (between 1-and 8-cell embryonic stages). Twelve of these transcripts wer e found to be initially expressed at 96 hr post-hCG injection (at 4-cell em bryonic stage) and beyond. Three of such genes were shown by sequence analy sis to be the porcine transforming growth factor-alpha, the porcine transfo rming growth factor-beta-binding protein II and a porcine astral natriureti c factor receptorlike transcript. Only one differentially expressed gene wa s detected between 50-60 and 85 hr post-hCG injection, and this gene turned out to be the porcine follicle-stimulating hormone receptor. The remaining eight transcripts detected by DDRT-PCR were novel. Moreover, most of these newly expressed genes were found to be turned on at a time coincidental wi th that of the 4-cell block of porcine embryos cultured in vitro. Our resul ts demonstrate that DDRT-PCR is a feasible approach for rapid identificatio n of genes that are differentially expressed in oviductal epithelium. Some of the genes thus identified may be important for unhindered development of embryos in the oviduct. Mol. Reprod. Dev. 56:331-335, 2000. (C) 2000 Wiley -Liss, Inc.