Increase of GAP-43 expression following kainic acid injection into whiskerbarrel cortex

Citation
T. Yamamoto et al., Increase of GAP-43 expression following kainic acid injection into whiskerbarrel cortex, NEUROREPORT, 11(8), 2000, pp. 1603-1605
Citations number
23
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROREPORT
ISSN journal
09594965 → ACNP
Volume
11
Issue
8
Year of publication
2000
Pages
1603 - 1605
Database
ISI
SICI code
0959-4965(20000605)11:8<1603:IOGEFK>2.0.ZU;2-V
Abstract
Plasticity after microinjection of kainic acid (KA) into the adult rat whis ker barrel cortex was investigated with immunohistochemical staining of pho sphorylated growth-associated protein (GAP)-43. After mapping the barrel co rtex with the technique of intrinsic signal optical imaging, a small volume of KA was injected into one barrel. Rats were sacrificed at 2 days, 3 days , 1 week, and 6 weeks after lesioning. GAP-43 staining demonstrated intense immunoreactivity (IR) at the injected barrel which spread to the inter-bar rel septa and the surrounding barrels. Elevated IR of GAP-43 was visible 2 days after KA injection, and increased gradually at least 6 weeks following the lesion. This model has the possibility of offering a simple and reliab le tool for studying cortical plasticity. NeuroReport 11:1603-1605 (C) 2000 Lippincott Williams & Wilkins.