Application of vanadium and nickel to the standard material for testing method of biocompatibility using cultured cells

The development of standard material to judge cell-culture technique and ce ll-response is requested in cytotoxicity testing methods for biocompatibili ty of biomaterials. Vanadium or nickel standard solutions used in inductive ly coupled plasma (ICP) analysis were added separately at appropriate conce ntrations to Eagle's medium and alpha-modified Eagle's medium (alpha-medium ). Vanadium or nickel particles were independently extracted in ultra-pure water and Eagle's medium. Then, these extracted solutions were added to fre sh Eagle's and alpha-mediums. Murine fibroblast L929 cell and murine osteob lastic MC3T3-E1 cells were respectively cultured with these Eagle's and alp ha-mediums. The effect of the vanadium and nickel ions in the standard solu tion used in ICP analysis on cell growth were good accordance with that of the extraction using vanadium and nickel particles. Therefore, it was thoug ht that vanadium or nickel standard solutions used in ICP analysis could be applied as a standard material in biocompatibility tests using cultured ce lls.