Photomechanical drug delivery into bacterial biofilms

Purpose. To investigate whether photomechanical waves generated by lasers c an increase the permeability of a biofilm of the oral pathogen Actinomyces viscosus. Methods. Biofilms of Actinomyces viscosus were formed on bovine enamel surf aces. The photomechanical wave was generated by ablation of a target with a a-switched ruby laser and launched into the biofilm in the presence of 50 mu g/ml methylene blue. The penetration depth of methylene blue was measure d by confocal scanning laser microscopy. Also, the exposed biofilms were ir radiated with light at 666 nm. After illumination, adherent bacteria were s craped and spread over the surfaces of blood agar plates. Survival fraction s were calculated by counting bacterial colonies. Results. Confocal scanning laser microscopy revealed that a single photomec hanical wave was sufficient to induce a 75% increase in the penetration dep th of methylene blue into the biofilm. This significantly increased the con centration of methylene blue in the biofilm enabling its photodestruction. Conclusions. Photomechanical waves provide a potentially powerful tool for drug delivery that might be utilized for treatment of microbial infections.