A. Ohara et al., Plant regeneration from hairy roots induced by infection with Agrobacterium rhizogenes in Crotalaria juncea L., PL CELL REP, 19(6), 2000, pp. 563-568
Hairy roots were induced from leaf segments of Crotalaria juncea, which is
used as a green manure crop antagonistic to nematodes, by infection with a
mikimopine type wild strain of Agrobacterium rhizogenes A13 (MAFF02-10266).
These roots exhibited vigorous growth and abundant lateral branching on ha
lf-strength Murashige and Skoog (1/2MS) medium without phytohormones. The a
dventitious shoots were induced from 30% of root segments 3 months after tr
ansfer onto medium containing 3 mg/l benzyl adenine. These shoots produced
roots 1 month after transfer onto 1.2% agar-solidified 1/2MS medium without
phytohormones. Regenerated plants were successfully grown under greenhouse
conditions. The transgenic nature of the regenerated plants was confirmed
by Southern-blot analysis.