In vitro pollination of isolated ovules of Cichorium intybus L.

Authors
Castano, CI De Proft, MP
Citation
Ci. Castano et Mp. De Proft, In vitro pollination of isolated ovules of Cichorium intybus L., PL CELL REP, 19(6), 2000, pp. 616-621
Citations number
24
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
07217714 → ACNP
Volume
19
Issue
6
Year of publication
2000
Pages
616 - 621
Database
ISI
SICI code
0721-7714(200005)19:6<616:IVPOIO>2.0.ZU;2-Q
Abstract
A method for the in vitro pollination of chicory (Cichorium intybus L.) ovu les was developed. The purpose was to avoid self-incompatibility after in v itro self-pollination of ovules isolated from flower buds before anthesis a nd from open flower heads. Seedlings were obtained at a low percentage (0.7 6%), and the results were explained in terms of pollen viability, pollen ge rmination on the ovule, embryogenesis studies and ploidy analysis.