J. Chen et al., Differences in cultivar response and complete sequence analysis of two isolates of wheat yellow mosaic bymovirus in China, PLANT PATH, 49(3), 2000, pp. 370-374
Seeds of selected European and Japanese winter wheat cultivars were grown a
t two experimental sites in China, namely Yaan, Sichuan province (YA), and
Yangzhou, Jiangsu province (YZ), where wheat yellow mosaic bymovirus (WYMV)
was severe. There were some differential responses of the cultivars to the
virus isolates present at the two sites. The complete nucleotide sequence
of both RNAs of both virus isolates was determined. Their genome organizati
on was identical to that reported for a Japanese isolate and the sizes were
very similar. Nucleotide comparisons demonstrated that parts of the CI and
NIa coding regions on RNA1 and the N-terminal part of the P2 coding region
on RNA2 were particularly variable, while substantially conserved regions
occurred in the 3' UTR of RNA2, the 7K, one part of the CI and parts of the
NIb and coat protein. It seems unlikely that differences in the 7K and NIa
-VPg proteins are responsible for virulence differences and the CI and NIb
regions were considered the most promising for further study.