TAR cloning of the human chromosome 7 short arm in yeast and screening forterminal sequences

Authors
Glazkova, DV Efimenko, IG Legchilina, SP Bornholdt, D Grzeschik, KH Arman, IP
Citation
Dv. Glazkova et al., TAR cloning of the human chromosome 7 short arm in yeast and screening forterminal sequences, RUSS J GEN, 36(5), 2000, pp. 502-508
Citations number
19
Categorie Soggetti
Molecular Biology & Genetics
Journal title
RUSSIAN JOURNAL OF GENETICS
ISSN journal
10227954 → ACNP
Volume
36
Issue
5
Year of publication
2000
Pages
502 - 508
Database
ISI
SICI code
1022-7954(200005)36:5<502:TCOTHC>2.0.ZU;2-U
Abstract
A partial clone library of the short arm of human chromosome 7 was created in yeast artificial chromosomes (YAC) using TAR cloning. The DNA of monochr omosome somatic hybrid cells (mouse/human) RuRag 14-4-7-44 containing short arm human chromosome 7 was used for cloning. The clone library was screene d for YACs with the human DNA; the mitotic stability of these YACs, the siz es of cloned fragments, and an independent clonal distribution in the chrom osome were determined. Human YACs were tested for the presence of chromosom e 7p telomeric sequences.