COMPARISON OF D3112 AND B3 TRANSPOSABLE P HAGE INSERTIONS LOCATED IN THE PSEUDOMONAS-AERUGINOSA CHROMOSOME

Preferential chromosomal integration sites (sites of conservative tran sposition) of transposable phage D3112 were identified by means of pul sed-field gel electrophoresis and separation of restriction fragments of the Pseudomonas aeruginosa chromosome. Phage B3 was shown to differ from D3112 with respect to this trait. High site-specificity and regi onal specificity, typical for conservative transposition of D3112, can partially explain the absence of mutations in biosynthetic P. aerugin osa genes upon cell lysogenization with D3112 phage (unlike B3 phage).