Influence of microcystine-YR and nodularin on the activity of some glucosidases in mouse liver

Microcystine-YR (20 ug/kg bodyweight) and 8 ug/kg bodyweight of nodularin w ere intraperitoneally injected to 90 female Swiss mice. After 15, 30, 60 mi n and 24 h the changes were observed in the activity of some glucosidases i n the complete cell homogenate and in the lysosomal, microsomal and cytosol fraction. Significant differences were connected with the time after admin istration of poison and with the cellular fraction. Nodularin induces the a ctivity of beta-D-glucuronidase, alpha-glucosidase, lysosomal esterase and N-acetyl-glucosaminidase and influenced on the labilisation of endoplasmati c reticulum membranes. Microcystine-YR inhibited the biosynthesis of glucos idases and revealed a destructive effect on membranes of lysosomes and endo plasmatic reticulum. (C) 2000 Elsevier Science Ireland Ltd. All rights rese rved.