Morphometry of the FRTL-5 cells after irradiation

The morphological changes of in vitro irradiated FRTL-5 cells and their abi lity to grow in semi-solid medium were studied morphometrically. FRTL-5 cel ls were grown in medium with 4 different concentrations of TSH (0, 0.1, 1, 10 mU/ml). After irradiation with 0 Gy, 2 Gy and 4 Gy, the cells were seede d on glass cover-slips and in methocel. Fourteen days after irradiation, th e morphometric analysis of FRTL5 cells and their nuclei was performed. The results showed that irradiation and different doses of TSH have influence o n FRTL-5 cell size, more on their nuclei than on the cells as a whole. Grow ing of FRTL-5 cells in the methocel indicates the possible transformation o f these cells after long-culturing in the TSH medium and after irradiation.