Adaptation of the gastric mucosa to nonsteroidal anti-inflammatory drug-ind
uced injury is a well-documented phenomenon, but the mechanisms are not kno
wn. We investigated whether changes in stress protein expression and apopto
sis play roles in adaptation of rat stomach to aspirin. RT-PCR and Western
blotting techniques were used to analyze mRNA and protein expression of HSP
72 and HSP90 and cleavage of caspase 3 protein. Apoptosis was detected by t
he TUNEL method and quantified. HSP72 mRNA and protein expression was uncha
nged in adapted mucosa, whereas HSP90 mRNA and protein levels decreased. Ca
spase 3 protein was activated, and the number of apoptotic cells increased
in mucosa after one aspirin dose. However, in adapted mucosa after aspirin,
activated caspase 3 and the number of apoptotic cells had returned to basa
l levels. Induction of the stress response was found not to be a mechanism
of mucosal adaptation against multiple doses of aspirin. Our results lead u
s to propose instead that resistance to aspirin-induced apoptosis plays a r
ole in the protective phenomenon of adaptation.