Xplore (R) mRNA assays for the quantification of IL-1 beta and TNF-alpha mRNA in lipopolysaccharide-induced mouse macrophages

Authors
Van Arsdell, SW Murphy, KP Pazmany, C Erickson, D Burns, C Moody, MD
Citation
Sw. Van Arsdell et al., Xplore (R) mRNA assays for the quantification of IL-1 beta and TNF-alpha mRNA in lipopolysaccharide-induced mouse macrophages, BIOTECHNIQU, 28(6), 2000, pp. 1220
Citations number
17
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOTECHNIQUES
ISSN journal
07366205 → ACNP
Volume
28
Issue
6
Year of publication
2000
Database
ISI
SICI code
0736-6205(200006)28:6<1220:X(MAFT>2.0.ZU;2-M
Abstract
Because the accurate measurement of a number of cytokine mRNA transcripts p rovides valuable knowledge about cytokine gene regulation, we have develope d the Xplore(R) assay for the quantification of cytokine mRNA. This micropl ate-based assay is rapid (under four hours), quantitative over three orders of magnitude and carries no risk of false-positive values from contaminati on with amplified target. Here, we describe the use of Xplore assays to mea sure the steady-state mRNA levels of TNF-alpha and IL-1 beta produced by mo use WEHI and J774 macrophage-like cell lines.