Characterization of electroosmotic flow in argentation capillary electrophoresis

A theoretical model for electroosmotic mobility (mu(eo)) in argentation cap illary electrophoresis has been established and the effects of buffer pH, A g(I) concentration, temperature and organic solvent on electroosmotic mobil ity investigated. The decrease in mu(eo) with increasing Ag(I) concentratio n is the sum of the decrease in the double layer thickness and the reductio n in the surface charge density due to the electrostatic interaction or che lation between Ag(I) and the charged surface groups. A characteristic disso ciation constant of 0.032 mM was obtained for the Ag(I)-siloxide complex fr om the experimental results. This constant is much smaller than the corresp onding value for the sodium ion.