Cysteine protease production by human osteosarcoma cells (MG63, SAOS2) andits modulation by soluble factors

The production of cysteine protease by two human osteosarcoma cell lines (M G-63 and SaOS2) was analyzed, as well as their modulation by interleukin 1 beta (hIL-1 beta), interleukin 6 (hIL-6), insulin growth factor-1 (hIGF-1), oncostatin M (hOSM), leukemia inhibitory factor (hLIF) and growth hormone (hGH), Cysteine protease activities were detected using a synthetic substra te. The protease activities (especially cathepsin L activity) of both cell lines were increased significantly in the presence of hIL-1 beta, hIL-6 and hOSM, In contrast, hIGF-1 and hGH decreased these activities, and no effec t was detectable in the presence of hLIF. The addition of antibodies agains t the gp-130 chain of the hIL-6 and hOSM receptors totally inhibited the st imulating effect of these two cytokines on cysteine protease activities. In increasing collagen type I degradation, hIL-1 beta, hIL-6 and hOSM could b e involved in bone resorption, whereas the inhibitory action of hIGF-1 and hGH on collagen type I degradation suggest that this factor could play a ro le in bone formation. (C) 2000 Academic Press.