RECOMBINANT ENV-GP85 OF HPRS-1OS (SUBGROUP-J) AVIAN-LEUKOSIS VIRUS - ANTIGENIC CHARACTERISTICS AND USEFULNESS AS A DIAGNOSTIC REAGENT

Authors
VENUGOPAL K HOWES K BARRON GS PAYNE LN
Citation
K. Venugopal et al., RECOMBINANT ENV-GP85 OF HPRS-1OS (SUBGROUP-J) AVIAN-LEUKOSIS VIRUS - ANTIGENIC CHARACTERISTICS AND USEFULNESS AS A DIAGNOSTIC REAGENT, Avian diseases, 41(2), 1997, pp. 283-288
Citations number
14
Categorie Soggetti
Veterinary Sciences
Journal title
Avian diseases → ACNP
ISSN journal
00052086
Volume
41
Issue
2
Year of publication
1997
Pages
283 - 288
Database
ISI
SICI code
0005-2086(1997)41:2<283:REOH(A>2.0.ZU;2-Z
Abstract
We describe the construction of a recombinant baculovirus containing t he cloned DNA encoding the gp85 envelope glycoprotein of HPRS-103 (sub group J) avian leukosis virus fused to the carboxy-terminus of the aff inity tag glutathione-S-transferase. The fusion protein was efficientl y secreted into the supernatant medium of the infected insect cell cul ture and could be purified in a single step using immobilized glutathi one. An enzyme-linked immunosorbent assay using the recombinant protei n was found to be specific and sensitive for detection of HPRS-103 vir us-specific antibodies in the sera of infected birds.