Efficacy of ELISA for detection of antibodies against several Ornithobacterium rhinotracheale serotypes

The present investigation were carried out to compare self-made ELISA based on SDS-Antigen extraction of serotype B and one commercial ELISA-kit (Bioc heck, Gouda, The Netherlands) for their ability to detect antibodies agains t 12 ORT serotypes (A-L). Using both ELISA systems, antibodies against all serotypes were detected. Examination of serum samples collected from commer cial flocks showed similar results on flock bases. However, some minor vari ations on sample basis could be demonstrated. In conclusion using both ELIS A system antibodies against different serotypes could be detected.