Background: The body's nucleotide pool derives from three potential sources
: de novo synthesis, salvage of preformednucleosides/bases or the diet. The
relative contributions of these pathways of assimilation are poorly unders
tood in vivo. Dietary nucleotides have been suggested Co have beneficial ef
fects an the development and repair of the gastrointestinal tract. Tissues
with a rapid turnover, such as the gut and the immune system cells, may uti
lise preformed nucleotides (coming from the diet), in situations in which t
here is a high demand of nucleotides for nucleic acid synthesis. Therefore,
nucleotides could be considered as conditionally essential nutrients.
Aim of the work and methods:Development of a method to measure synthesis de
novo of RNA-purine nucleotides in Caco-2 cells, relying an the incorporati
on of C-14-glycine into the purine ring of the nucleotide.
To establish the fractional synthesis race of RNA purine nucleotides in Cac
o-2 cells, grown in culture medium containing different concentrations of g
lutamine, in the presence or abscence of added nucleotides.
To investigate the degree to which tissue ribonucleosides are derived from
the culture medium or from de novo synthesis in the presence of different c
oncentrations of glutamine, using undifferentiated Caco-2 cells, stressed o
r not by the addition of IL-1 beta to the medium.
Results and conclusions: The presence of high levels of glutamine in the cu
lture medium is essential for cell proliferation (estimated by measurement
of the fractional synthesis rate of purine nucleotides) and the presence of
nucleotides cannot replace the glutaminedependence of Caco-2 cell prolifer
ation. The incorporation of exogenous purine nucleotides into RNA of Caco-2
cells is rather limited, and it becomes important when cells are stressed
by glutamine deprivation.
Stress by addition of interleukin-1 beta resulted in the maintenance or the
increase in de novo synthesised RNA-purine nucleotides, even in the presen
ce of exogenous nucleotides. However, the addition of interleukin-1 beta to
the culture medium led to an enhanced salvage of preformed pyrimidine nucl
eotides for nucleic acid synthesis when glutamine was present in the medium
at a concentration of 0.5 mmol/L.