Objective: To review 35 cases of preimplantation genetic diagnosis (PGD) of
translocations with several methods, including telomeric probes.
Design: Retrospective study.
Setting: Clinical IVF laboratory.
Patient(s): Thirty-five couples with one partner carrying a chromosomal tra
nslocation.
Intervention(s): PGD of translocation after polar-body or embryo biopsy.
Main Outcome Measure(s): Pregnancy outcome.
Result(s): Several trends were observed. First, PGD can achieve a statistic
ally significant reduction in spontaneous abortion, from 95% to 13%. Second
, the chances of achieving pregnancy are correlated with 50% or more of the
embryos being chromosomally normal. Third, patients with robertsonian tran
slocations produced fewer abnormal gametes and more pregnancies than did pa
tients with reciprocal translocations. Fourth, a new fluorescence in situ h
ybridization protocol for PGD of translocations, which involves applying te
lomeric probes, has proved adequately reliable with a 6% average error rate
.
Conclusion(s): PGD of translocations achieves a statistically significant r
eduction in spontaneous abortion, both for polar-body and blastomere biopsy
cases. Pregnancy outcome depended on the number of normal embryos availabl
e for transfer, with patients having <50% abnormal embryos achieving the mo
st pregnancies. Because robertsonian translocations caused fewer abnormal e
mbryos than reciprocal translocations, they also resulted in higher rates o
f implantation. ((C) 2000 by American Society for Reproductive Medicine.)