Extraction, purification and preparation of species-specific phenolic glycolipid (PGL) from Mycobacterium bovis (AN5)

Authors
Nayak, S Verma, R Kumari, N Venkatesan, K
Citation
S. Nayak et al., Extraction, purification and preparation of species-specific phenolic glycolipid (PGL) from Mycobacterium bovis (AN5), I J ANIM SC, 70(5), 2000, pp. 446-448
Citations number
19
Categorie Soggetti
Animal Sciences
Journal title
INDIAN JOURNAL OF ANIMAL SCIENCES
ISSN journal
03678318 → ACNP
Volume
70
Issue
5
Year of publication
2000
Pages
446 - 448
Database
ISI
SICI code
0367-8318(200005)70:5<446:EPAPOS>2.0.ZU;2-E
Abstract
Phenolic glycolipid (PGL) which is species-specific to Mycobacterium bovis AN5, was extracted, purified and deacylated using preparative thin layer ch romatography and florisil column. On single directional chromatography, usi ng solvent system chloroform : methanol : water (90:10:1, v/v x 1), the mob ility of deacylated PGL of M. bovis AN5 was identical with the mobility of authentic deacylated PGL (Rf value 0.23).