Evaluation of a Yersinia adhesion gene (yadA) specific PCR for the identification of enteropathogenic Yersinia enterocolitica

Authors
Neubauer, H Hensel, A Aleksic, S Meyer, H
Citation
H. Neubauer et al., Evaluation of a Yersinia adhesion gene (yadA) specific PCR for the identification of enteropathogenic Yersinia enterocolitica, INT J F MIC, 57(3), 2000, pp. 225-227
Citations number
17
Categorie Soggetti
Food Science/Nutrition
Journal title
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
ISSN journal
01681605 → ACNP
Volume
57
Issue
3
Year of publication
2000
Pages
225 - 227
Database
ISI
SICI code
0168-1605(20000615)57:3<225:EOAYAG>2.0.ZU;2-L
Abstract
A total of 101 Yersinia enterocolitica strains was investigated with a PCR assay [Blais and Phillipe, Food Control, 6 (1995) 211-214] targeting the Ye rsinia adhesin gene (yadA) responsible for autoagglutination. Compared to t he autoagglutination test the PCR assay has a specificity of 100% but a sen sitivity of only 70%. This failure might be caused by the sequence heteroge neity of yadA. (C) 2000 Elsevier Science B.V. All rights reserved.