Volume expansion sensitivity of swelling-activated Cl- channel in human epithelial cells

In human epithelial cell lines, whole-cell swelling-activated Cl- current w as not directly correlated to cell volume per se, membrane tension or hydro static pressure. The current density exhibited a relation described by a Bo ltzmann function to the square of the cell diameter. Cytochalasin D enhance d the volume sensitivity, These results suggest that the activation mechani sm of volume-sensitive Cl- channel is related to cytoskeleton-dependent mem brane spring energy in human epithelial cells.