A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model

Authors
McLachlan, CS Yin, JL Driussi, C Jusuf, PR Hambly, B McGuire, MA
Citation
Cs. Mclachlan et al., A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model, JPN J PHYSL, 50(2), 2000, pp. 281-284
Citations number
3
Categorie Soggetti
Physiology
Journal title
JAPANESE JOURNAL OF PHYSIOLOGY
ISSN journal
0021521X → ACNP
Volume
50
Issue
2
Year of publication
2000
Pages
281 - 284
Database
ISI
SICI code
0021-521X(200004)50:2<281:ASPMFT>2.0.ZU;2-W
Abstract
Cells that are apoptotic and comprise less than 2% of the total cellular po pulation are difficult to detect by conventional methods (i.e., DNA ladder) , We discuss a new methodological technique, PCR-amplified DNA ladder, to d etect very low levels of DNA fragmentation (indicative of apoptosis) in a m yocardial infarct heart failure model. Results and methodology are contrast ed with the traditional DNA ladder technique.