COLLECTION OF PANCREATIC-JUICE FROM GROWING PIGS - A COMPARATIVE-STUDY OF THE POUCH METHOD AND THE CATHETER METHOD

Conclusion. The results of this study demonstrate that there are large differences in the amount of pancreatic juice secreted and in the che mical and enzymatic composition of pancreatic juice when the pouch and the catheter methods were used, and these differences must be taken i nto consideration in future studies with either method. Methods. A stu dy was performed to compare the two most commonly used methods to coll ect pancreatic juice from growing pigs; namely, the pouch method (PM) and the catheter method (CM). In the first part of the study, three ba rrows (initial weight 37 kg) were fitted with a pancreatic pouch re-en trant cannula. An isolated pouch was prepared in which the pancreatic duct enters the duodenum. In the second part of the study, also with t hree barrows (initial weight 32 kg), a catheter was inserted into the pancreatic duct. Results. At several points during the 24-h collection , the hourly rate of pancreatic juice secretion in CM pigs was larger (p < 0.05) than for PM pigs. CM pigs also had a higher (p < 0.05) dail y volume of secretion, 4.09 vs 2.63 L/24 h for PM pigs. The pH of panc reatic juice collected from CM pigs was consistently higher (p < 0.01) throughout the 24-h collection. In contrast, the concentration and da ily output of bicarbonate did not differ between CM and PM pigs. The c oncentration of protein in pancreatic juice from PM pigs (7.21 gi L) w as higher (p < 0.001) than for CM pigs (4.08 g/L). Specific amylase an d lipase and total amylase activities were greater (p < 0.01) in pancr eatic juice collected from PM pigs. Specific and total carboxyl ester hydrolase and colipase activities were substantially (p < 0.01) larger in pancreatic juice collected from CM pigs. A major difference betwee n the methods was that trypsin and chymotrypsin were fully active in p ancreatic juice from PM pigs, whereas virtually no trypsin or chymotry psin activity was detected in pancreatic juice from CM pigs. Specific and total chymotrypsin activities did not differ between PM and CM pig s. Both specific and total trypsin activities were substantially highe r in pancreatic juice from CM pigs: 3682 U/L and 12,752 U/24 h, respec tively, vs 1031 U/L and 2639 U/24 h, respectively, in pancreatic juice from PM pigs.