Ms. Jensen et al., COLLECTION OF PANCREATIC-JUICE FROM GROWING PIGS - A COMPARATIVE-STUDY OF THE POUCH METHOD AND THE CATHETER METHOD, International journal of pancreatology, 21(2), 1997, pp. 173-184
Conclusion. The results of this study demonstrate that there are large
differences in the amount of pancreatic juice secreted and in the che
mical and enzymatic composition of pancreatic juice when the pouch and
the catheter methods were used, and these differences must be taken i
nto consideration in future studies with either method. Methods. A stu
dy was performed to compare the two most commonly used methods to coll
ect pancreatic juice from growing pigs; namely, the pouch method (PM)
and the catheter method (CM). In the first part of the study, three ba
rrows (initial weight 37 kg) were fitted with a pancreatic pouch re-en
trant cannula. An isolated pouch was prepared in which the pancreatic
duct enters the duodenum. In the second part of the study, also with t
hree barrows (initial weight 32 kg), a catheter was inserted into the
pancreatic duct. Results. At several points during the 24-h collection
, the hourly rate of pancreatic juice secretion in CM pigs was larger
(p < 0.05) than for PM pigs. CM pigs also had a higher (p < 0.05) dail
y volume of secretion, 4.09 vs 2.63 L/24 h for PM pigs. The pH of panc
reatic juice collected from CM pigs was consistently higher (p < 0.01)
throughout the 24-h collection. In contrast, the concentration and da
ily output of bicarbonate did not differ between CM and PM pigs. The c
oncentration of protein in pancreatic juice from PM pigs (7.21 gi L) w
as higher (p < 0.001) than for CM pigs (4.08 g/L). Specific amylase an
d lipase and total amylase activities were greater (p < 0.01) in pancr
eatic juice collected from PM pigs. Specific and total carboxyl ester
hydrolase and colipase activities were substantially (p < 0.01) larger
in pancreatic juice collected from CM pigs. A major difference betwee
n the methods was that trypsin and chymotrypsin were fully active in p
ancreatic juice from PM pigs, whereas virtually no trypsin or chymotry
psin activity was detected in pancreatic juice from CM pigs. Specific
and total chymotrypsin activities did not differ between PM and CM pig
s. Both specific and total trypsin activities were substantially highe
r in pancreatic juice from CM pigs: 3682 U/L and 12,752 U/24 h, respec
tively, vs 1031 U/L and 2639 U/24 h, respectively, in pancreatic juice
from PM pigs.