Sp. Felton et al., ASCORBYL-2-SULFATE COMPARED WITH ASCORBIC-ACID IN ATLANTIC SALMON - UPTAKE AND DISTRIBUTION CONFIRMED BY MASS-SPECTROSCOPY, Proceedings of the Society for Experimental Biology and Medicine, 215(3), 1997, pp. 248-257
This paper reports an in-depth approach that identifies ascorbyl-2-sul
fate (AS) in gastric, blood, liver, and muscle tissues of Atlantic sal
mon (Salmo salar), To insure the identity of the AS, the study utilize
d the latest high-performance liquid chromatography (HPLC) technology
plus electron ionization mass spectrometry (EIMS), Just before saltwat
er adaptation stage, juvenile Atlantic salmon were force-fed AS, ascor
bic acid (AA), and a molecular-equivalent combination of the two, Afte
r tissue analyses for AA and AS were performed by HPLC separation, the
HPLC peaks were identified by EIMS, The data collected in this study
indicate that Atlantic salmon can absorb AS through the gastric tissue
when forced-fed AA and AS as described, The data also indicate that A
S is transported through the blood to the liver, There is evidence to
indicate that AS is converted to AA In the livers of these salmon, In
addition, the muscle tissue contained a large portion of AA and AS.