Mr. Terebiznik et al., Combined effect of nisin and pulsed electric fields on the inactivation ofEscherichia coli, J FOOD PROT, 63(6), 2000, pp. 741-746
The Doehlert design was applied in order to investigate the combined effect
of nisin and high voltage pulsed electric fields (PEF) on the inactivation
of Escherichia coli in simulated milk ultrafiltrate media. Nisin alone was
totally inactivated by PEF, but in the presence of bacterial cells a prote
ctive effect was observed. However, the effectiveness of nisin was still de
creased when bacterial cells were subjected to the combined treatment. In s
pite of this phenomenon, an almost additive response emerged as a consequen
ce of the combined treatment. A 4-log cycle reduction may be accomplished w
ith around 1,000 IU/ml (7.15 mu M) of nisin and three pulses of 11.25 kV/cm
or 500 IU/ml for five pulses of the same intensity. The observed efficacy
arising from the combination of both treatments suggests the possibility of
using PEF for improving the action spectrum of natural antimicrobials.