Identification of species origin of fresh, cooked and decomposed meats using brain antigens

Antisera were raised in rabbits against fresh and heated brain antigens of cattle, buffalo, sheep, goat and pig as well as those of rabbit fresh and r abbit thermostable brain antisera were raised in sheep and goat, respective ly. These antisera were used to identify the species origin of fresh, cooke d and decomposed meats, by conducting double immunodiffusion (DID), immunoe lectrophoresis (IE) and counter immunoelectrophoresis (CIE) techniques. All the antisera. developed cross reactions except sheep anti-rabbit native, a nd goat anti-rabbit thermostable brain antisera and, rabbit anti-pig native as well as thermostable antisera. which proved species-specific. All the c ross-reacting antisera after successful absorption and other natural specie s specific antisera were used to detect adulterated meats either fresh or c ooked and also decomposed.