Peroxisome proliferator-activated receptor-gamma activators inhibit IFN-gamma-induced expression of the T cell-active CXC chemokines IP-10, Mig, and I-TAC in human endothelial cells

Peroxisome proliferator-activated receptor-gamma (PPAR gamma), a member of the nuclear hormone receptor superfamily originally shown to play an import ant role in adipocyte differentiation and glucose homeostasis, is now known to regulate inflammatory responses. Given the importance of endothelial ce ll (EC)-derived chemokines in regulating leukocyte function and trafficking , we studied the effects of PPAR gamma ligands on the expression of chemoki nes induced in ECs by the Th1 cytokine IFN-gamma, Treatment of ECs with PPA R gamma activators significantly inhibited IFN-gamma-induced mRNA and prote in expression of the CXC chemokines IFN-inducible protein of 10 kDa (IP-10) , monokine induced by IFN-gamma (Mig), and IFN-inducible T-cell alpha-chemo attractant (I-TAC), whereas expression of the CC chemokine monocyte chemoat tractant protein-1 was not altered. PPAR gamma activators decreased IFN-ind ucible protein of 10 kDa promoter activity and inhibited protein binding to the two NF-kappa B sites but not to the IFN-stimulated response element IS RE site. Furthermore, PPAR gamma ligands inhibited the release of chemotact ic activity for CXC chemokine receptor 3 (CXCR3)-transfected lymphocytes fr om IFN-gamma-stimulated ECs, These data suggest that anti-diabetic PPAR gam ma activators might attenuate the recruitment of activated T cells at sites of Th1-mediated inflammation.