Oncolytic viral therapy is a promising new method of cancer treatment. Peri
toneal dissemination of cancer is a common and fatal clinical condition see
n in many malignancies, with few effective therapies available. G207, a mul
timutated replication-competent herpes simplex virus type-1, effectively tr
eats disseminated peritoneal cancer This study evaluates viral proliferatio
n and subsequent tumoricidal effects in vitro and in vivo after regional vi
ral delivery. In vitro studies demonstrate that G207 efficiently kills five
human gastric cancer cell lines, and that permissiveness to viral replicat
ion is correlated with cytotoxicity. In a murine xenograft model of human g
astric carcinomatosis, peritoneal delivery of G207 effectively kills tumor
and prolongs survival. Data from quantitative PCR characterizes peritoneal
clearance of virus after intraperitoneal injection, and identifies G207 rep
lication within tumor cells in vivo, similar to in vitro proliferation. Fur
ther analysis of various organs confirms that G207 does not replicate withi
n normal tissue after peritoneal delivery. Wild-type KOS viral replication
was also demonstrated in vivo, with significant toxicity secondary to disse
mination and encephalitis. In vivo viral proliferation of G207 is restricte
d to tumor cells, is correlated with in vitro assays, and is an important m
echanism of anticancer efficacy.