R. Kalawski et al., Stimulation of neutrophil integrin expression during coronary artery bypass grafting: Comparison of crystalloid and blood cardioplegic solutions, J THOR SURG, 119(6), 2000, pp. 1270-1277
Citations number
30
Categorie Soggetti
Cardiovascular & Respiratory Systems","Cardiovascular & Hematology Research
Objectives: This study was designed (1) to evaluate the influence of plasma
obtained from patients undergoing coronary artery bypass grafting on L-sel
ectin, CD11b, and CD18 expression on human neutrophils and (2) to determine
the influence of the use of crystalloid or blood cardioplegia during bypas
s grafting on plasma-mediated expression of adhesion molecules on polymorph
onuclear neutrophils,
Patients and methods: Patients undergoing coronary artery bypass grafting w
ere divided into 2 groups to receive crystalloid or blood cardioplegic solu
tions. Peripheral vein, radial artery, and coronary sinus blood samples wer
e drawn at aortic crossclamping, aortic crossclamp release, and 30 minutes
after reperfusion. Human neutrophils were incubated with patients' plasma,
and the expression of CD11b, CD18, and L-selectin was determined with flow
cytometry.
Results: In patients receiving crystalloid cardioplegic solutions, plasma s
amples collected from the coronary sinus at aortic clamp release and 30 min
utes thereafter induced significantly higher expression of neutrophil CD11b
and CD18 than plasma samples obtained from a peripheral vein or artery at
the same time points. The expression of L-selectin on polymorphonuclear neu
trophils was significantly reduced with plasma obtained 30 minutes after re
perfusion as compared with samples collected at aortic crossclamp release.
In the group receiving blood cardioplegia, no significant differences in CD
11b, CD18, or L-selectin expression were found.
Conclusions: (1) Ischemia/reperfusion after coronary artery bypass grafting
is associated with the release of factors capable of neutrophil activation
from myocardium into the circulating blood. (2) The release of soluble sti
muli for neutrophils during bypass grafting may be modified by the cardiopl
egic solution.