Because of weak resistance of canine sperm to freezing, an applicable metho
d of preparing canine frozen semen has not yet been established. We added v
arious concentrations of Orvus ES Paste (OEP) to egg yolk Tris-fructose cit
rate, and investigated its effectiveness on survival of spermatozoa. Additi
on of 0.5-1.0% OEP to the extender for freezing canine semen was effective
in prolonging post-thaw survival of spermatozoa.