Soft-lithography-mediated submicrometer patterning of self-assembled monolayer of hemoglobin on ITO surfaces

The recent direct fabrication of micropatterned arrays of active biomolecul es has received overwhelming attention due to its potential applications in biosensors and medical diagnostics. Here, we report an indirect soft-litho graphy-mediated method of fabricating hemoglobin (Hb) patterns in the submi crometer length scales region on indium-tin-oxide (ITO). Scanning electron microscopy, quartz crystal microbalance, and cyclic voltammetry studies rev eal the formation of a self-assembled monolayer of Hb on an ITO surface. Th e present approach provides an easy and reliable means for simultaneous and defectless passivation and submicrometer patterning of proteins, thus indi cating its potential application in biological assay.