Cd. Kang et al., The inhibition of ERK/MAPK not the activation of JNK/SAPK is primarily required to induce apoptosis in chronic myelogenous leukemic K562 cells, LEUK RES, 24(6), 2000, pp. 527-534
in this study, the downstream signaling of Bcr-Abl tyrosine kinase responsi
ble for apoptosis resistance was investigated. DNA fragmentation, a hallmar
k of apoptosis, was observed after 2 days of herbimycin A treatment with a
peak on 3 day. During the apoptosis induced by the treatment of herbimycin
A, stress-activated protein kinase (SAPK) and p38 kinase were activated tim
e- and dose-dependently, while extracellular signal-regulated kinase (ERK)
was inhibited. However, apoptosis was induced by the treatment of PD98059,
a specific inhibitor of MEK (MAPK or ERK kinase), not by the treatment of s
orbitol, a strong activator of SAPK and p38 kinase. Although K562 cells wer
e very resistant to sorbitol-induced apoptosis, DNA fragmentation was induc
ed rapidly in Jurkat, HL-60 and U937 cells after exposure to sorbitol, desp
ite that these apoptosis-sensitive cells have similar or lower activities o
f JNK/SAPK and p38 kinase compared with K562 cells after treatment of sorbi
tol. K562 cells had a much higher basal activity of ERK/MAPK than other apo
ptosis-sensitive cell lines, which were very susceptible to apoptosis induc
ed by low dose of PD98059 compared with K562 cells. In HL-60 cells, sorbito
l-induced apoptosis was prevented by the treatment of phorbol myristate 13-
acetate (PMA), which activates the ERK/MAPK pathway, and this was blocked b
y PD98059. From these results, it could be suggested that the inhibition of
ERK/MAPK not the activation of JNK/SAPK is primarily required to induce ap
optosis in K562 cells. (C) 2000 Published by Elsevier Science Ltd. All righ
ts reserved.