DNA in situ hybridization on polytene chromosomes of Simulium sanctipauli at loci relevant to insecticide resistance

Authors
Boakye, DA Cornel, AJ Meredith, SE Brakefield, PM Collins, FH
Citation
Da. Boakye et al., DNA in situ hybridization on polytene chromosomes of Simulium sanctipauli at loci relevant to insecticide resistance, MED VET ENT, 14(2), 2000, pp. 217-222
Citations number
28
Categorie Soggetti
Entomology/Pest Control
Journal title
MEDICAL AND VETERINARY ENTOMOLOGY
ISSN journal
0269283X → ACNP
Volume
14
Issue
2
Year of publication
2000
Pages
217 - 222
Database
ISI
SICI code
0269-283X(200006)14:2<217:DISHOP>2.0.ZU;2-H
Abstract
A DNA technique for in situ hybridization developed by Kumar & Collins (199 4) for use on polytene chromosomes of adult Anopheles mosquitoes (Diptera: Culicidae) was modified for use with Simulium larval salivary gland chromos omes (Diptera: Simuliidae). Cloned fragments of several Simulium genes (cod ing for aspartate amino transferase, cytochrome P450 and DNA polymerase) we re successfully mapped physically by assigning specific band locations in S imulim sanctipauli V. & D. This represents the first attempt at locating ge nes beyond the resolution of linkage to inversions in any blackfly species.