Increased high-density lipoprotein-3 binding to leukocytes following weight loss and improved glycemic control in type 2 diabetic patients

This study evaluates the effect on high-density lipoprotein (HDL) binding a ctivity in cultured granulocytes before and after metabolic control of non- insulin-dependent diabetes mellitus ([NIDDM] type 2 diabetes) patients. In 20 type 2 diabetic patients, diabetic control was accomplished by administr ation of oral antidiabetic agents and dietary restrictions. Adequate metabo lic control was reflected by a decrease in the fasting glucose, glycosylate d hemoglobin (HbA(1c)), mean insulin, and body mass index (BMI). After cont rol of the diabetes, the mean HDL3 cholesterol was increased from 0.918 +/- 0.05 to 1.008 +/- 0.05 mmol/L (P < .05) and apolipoprotein Al (apo Al) was increased from 103 +/- 5.8 to 115 +/- 5.1 mg/dL (P < .01). The HDL3 maximu m specific binding was higher after versus before diabetic control, 77 +/- 6 versus 122 +/- 8 ng/mg cell protein (P < .01). This increase was related to an increase in maximum binding ([B-max] from 4.97 x 10(-10) to 8.3 x 10( -10) mol/L, P < .001), and no significant changes were observed in the K-d (from 1.47 x 10(-7) v 2.04 x 10(-7) mol/L). These results suggest that the metabolic control of type 2 diabetes increases HDL3 binding activity. Copyr ight (C) 2000 by W.B. Saunders Company.