The maize streak virus coat protein transcription unit exhibits tissue-specific expression in transgenic rice

Maize streak geminivirus (MSV) is a single-stranded DNA virus that infects cereals and other grasses. A promoter region incorporating the MSV large in tergenic region and movement protein gene sequence was ligated to the gus ( beta-glucuronidase) reporter gene which replaced the virus coat protein (CP ) gene. The CP promoter activity was analysed in transgenic rice plants (Or yza sativa L.) and was compared with that obtained in plants transformed wi th the gus gene downstream of the cauliflower mosaic virus (CaMV) 35S promo ter. The MSV CP promoter activity varied in the five plant lines tested, bu t was always less than that of the CaMV promoter. Histochemistry showed tha t the MSV CP promoter was active in cells of regenerating callus but in reg enerated plants it provided an expression pattern restricted to the vascula r tissues of the root, stem, leaf and floral organs. Expression was highest in phloem-associated tissues of the vegetative organs and was absent from the tip and elongation region of seedling roots. Thus, the MSV CP promoter shows a degree of developmental regulation and can be used to confer tissue -specific expression in transgenic rice plants. (C) 2000 Elsevier Science I reland Ltd. All rights reserved.