Anopheles gambiae pilot gene discovery project: Identification of mosquitoinnate immunity genes from expressed sequence tags generated from immune-competent cell lines

Together with AIDS and tuberculosis, malaria is at the top of the list of d evastating infectious diseases. However, molecular genetic studies of its m ajor vector, Anopheles gambiae. are still quite limited. We have conducted a pilot gene discovery project to accelerate progress in the molecular anal ysis of vector biology, with emphasis on the mosquito's antimalarial immune defense. A total of 5,925 expressed sequence tags were determined from nor malized cDNA libraries derived from immune-responsive hemocyte-like cell li nes. The 3,242 expressed sequence tag-containing cDNA clones were grouped i nto 2,380 clone clusters. potentially representing unique genes. Of these, 1,118 showed similarities to known genes from other organisms, but only 27 were identical to previously known mosquito genes. We identified 38 candida te genes, based on sequence similarity, that may be implicated in immune re actions including antimalarial defense; 19 of these were shown experimental ly to be inducible by bacterial challenge, lending support to their propose d involvement in mosquito immunity.