The system of regeneration and gene transformation of Rhaponticum carthamoides plants accumulating ecdysteroids

Authors
Orlova, IV Semenyuk, EG Volodin, VV Nosov, AM Bur'yanov, YI
Citation
Iv. Orlova et al., The system of regeneration and gene transformation of Rhaponticum carthamoides plants accumulating ecdysteroids, RUSS J PL P, 47(3), 2000, pp. 355-359
Citations number
25
Categorie Soggetti
Plant Sciences
Journal title
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
ISSN journal
10214437 → ACNP
Volume
47
Issue
3
Year of publication
2000
Pages
355 - 359
Database
ISI
SICI code
1021-4437(200005/06)47:3<355:TSORAG>2.0.ZU;2-1
Abstract
An efficient system for plant regeneration from leaf explants has been work ed out. Gene transformation of a medicinal plant Rhaponticum carthamoides ( Willd.) Iljin with a recombinant strain of Agrobacterium tumefaciens GV 310 1 containing plasmid with the gene rolC was performed for the first time. P CR analysis of DNA from transformed shoots showed that the genome comprised the copies of rolC and nptII genes.