H1 histamine receptor antagonist inhibits constitutive growth of Jurkat T cells and antigen-specific proliferation of ovalbumin-specific murine T cells
Z. Radvany et al., H1 histamine receptor antagonist inhibits constitutive growth of Jurkat T cells and antigen-specific proliferation of ovalbumin-specific murine T cells, SEM CANC B, 10(1), 2000, pp. 41-45
Histamine is produced from histidine by histidine decarboxylase (HDC) in ma
ny cells including normal and malignant lymphocytes. We examined the expres
sion of HDC and the effect of histamine receptor antagonists on the prolife
ration of a human T cell line, Jurkat and on antigen-driven proliferation o
f lymphocytes from ovalbumin-immunized mice. Our results demonstrate that H
DC is inducible in Jurkat cells by anti-CD3. The H1 receptor antagonist tri
prolidine dose dependently inhibits proliferation of both Jurkat cells and
ovalbumin-stimulated murine lymphocytes, while the H2 antagonist ranitidine
was ineffective. Alpha-fluoro-methyl-histidine blocking HDC activity did n
ot inhibit the T cell proliferation, suggesting an existing pool of histami
ne in T cells.