Phylogenetic placement of Puelia and Guaduella, two broad-leaved African gr
ass genera traditionally classified as bamboos, is inferred from analysis o
f ndhF rbcL, and PHYB sequence data. Puelia and Guaduella are supported as
sister genera, and the Puelia/Guaduella clade is resolved as an early diver
ging branch within the Poaceae and sister to the BEP + PACC clades, which i
nclude the bulk of the family. The new grass subfamily Puelioideae is descr
ibed to accommodate the Puelia/Guaduella clade.